The recent increase in unconventional oil and gas exploration and production has prompted a large amount of research on hydraulic fracturing, but the majority of chemical reactions between shale minerals and organic matter with fracturing fluids are not well understood. Organic matter, primarily in the form of kerogen, dominates the transport pathways for oil and gas; thus any alteration of kerogen (both physical and chemical properties) upon exposure to fracturing fluid may impact hydrocarbon extraction. In addition, kerogen is enriched in metals, making it a potential source of heavy metal contaminants to produced waters. In this study, we reacted two different kerogen isolates of contrasting type and maturity (derived from Green River and Marcellus shales) with a synthetic hydraulic fracturing fluid for 2 weeks in order to determine the effect of fracturing fluids on both shale organic matter and closely associated minerals. ATR-FTIR results show that the functional group compositions of the kerogen isolates were in fact altered, although by apparently different mechanisms. In particular, hydrophobic functional groups decreased in the Marcellus kerogen, which suggests the wettability of shale organic matter may be susceptible to alteration during hydraulic fracturing operations. About 1% of organic carbon in the more immature and Type I Green River kerogen isolate was solubilized when it was exposed to fracturing fluid, and the released organic compounds significantly impacted Fe oxidation. On the basis of the alteration observed in both kerogen isolates, it should not be assumed that kerogenic pores are chemically inert over the time frame of hydraulic fracturing operations. Shifts in functional group composition and loss of hydrophobicity have the potential to degrade transport and storage parameters such as wettability, which could alter hydrocarbon and fracturing fluid transport through shale. Additionally, reaction of Green River and Marcellus kerogen isolates with low pH solutions (full fracturing fluid, which contains hydrochloric acid, or pH 2 water) mobilized potential trace metal(loid) contaminants, primarily S, Fe, Co, Ni, Zn, and Pb. The source of trace metal(loid)s varied between the two kerogen isolates, with metals in the Marcellus shale largely sourced from pyrite impurities, whereas metals in the Green River shale were sourced from a combination of accessory minerals and kerogen.