In this paper, fungus hyphae/graphene oxide (FH/GO) with interesting net structure was prepared through a simple biological culture method for U(VI) removal. SEM, Zeta potential, FT-IR and XPS were applied to characterize the physicochemical properties of the resulting FH/GO composites, and batch and column adsorption experiments were conducted to elucidate their U(VI) adsorption performances and mechanisms. Results showed that FH/GO composite was highly efficient in U(VI) adsorption with a high reusability and stability. Specifically, compared to biomass (FH), the U(VI) adsorption capacity was increased by 60% for FH/GO composite at initial pH 6.0 (+/- 0.1) and 20 degrees C, and the maximum U(VI) adsorption capacity for the FH/GO was 199.37 mg/g. Furthermore, the adsorption kinetics, thermodynamics and isotherms analysis demonstrated that the U(VI) adsorption on FH/GO was depended on the chemical adsorption, and it was an endothermic and spontaneous process. Therefore, these results suggested that the as-prepared FH/GO composite was promising in nuclear waste water treatment.