클러스터 분석을 이용한 황련 추출물 항산화 활성 최적화 추출공정

부민민; 현송화; 박수남; Minmin Fu; Song Hua Xuan; Soo Nam Park

Applied Chemistry for Engineering, Vol.29, No.5, 604-612, October, 2018

DOI10.14478/ace.2018.1065 Export Citation

클러스터 분석을 이용한 황련 추출물 항산화 활성 최적화 추출공정

Optimization of the Extraction Process and Antioxidant Capacity of Coptis chinensis Franch Extract through Cluster Analysis

부민민, 현송화, 박수남 ^†

서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소

Minmin Fu, Song Hua Xuan, and Soo Nam Park^†

Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea

E-mail:

초록

황련은 여러 가지 질병 치료에 사용되는 효과적인 전통약용식물이다. 하지만 황련 추출물의 생리활성성분 함량에 영향을 미치는 주요 인자 및 생리활성성분과 항산화 효능의 상관관계는 아직 알려지지 않았다. 따라서 본 논문에서는 용매, 온도 및 추출 시간이 황련 추출물의 생리 활성성분의 함량과 항산화 활성에 미치는 영향을 조사하였다. 클라스터를 이용하여 분석한 결과, 35 ℃에서 추출시간 30 min인 에탄올(50% 에탄올 : 50% 물) 조건으로 추출 시, 생리활성 성분 함량 및 항산화 활성이 제일 높으므로 공장에서 사용하기에 가장 적합한 추출조건임을 확인하였다. 다중선형회귀분석 결과, 총 페놀화합물 함량은 DPPH소거 활성에 기여하였으며 총 알칼로이드 함량과 총 플라보노이드 함량은 FRAP활성에 기여하였다. 이상의 결과들로부터 황련의 추출 조건이 생리 활성 화합물의 수율 및 항산화 활성을 조절 가능하며, 이는 공업적인 응용에 중요한 정보를 제공할 수 있을 것으로 사료된다.

Coptis chinensis Franch is a valuable traditional oriental medicinal plant used for the treatment of various diseases. The major factors affecting the content of bioactive compounds and the relationship between bioactive compounds and antioxidant capacities of Coptis chinensis Franch were poorly understood. Thus, effects of the solvent, temperature, and extraction time on the extraction yields of bioactive compounds and the antioxidant activity of C. chinensis Franch extracts were investigated in this work. Our cluster analysis indicated that the hydroalcoholic solvent (50% ethanol : 50% water) at 35 ℃ for 30 min (extract time) was the best extraction condition for a factory use because the highest level of bioactive compounds and antioxidant activities was achieved. Multiple linear regression analysis revealed that total phenolic content (TPC) contributed to the 1,1-diphenyl- 2-picrylhydrazyl radical (DPPH) scavenging activity, while both total alkaloid content (TAC) and total flavonoid content (TFC) were responsible for ferric reducing antioxidant power (FRAP) activity. These results indicated that extraction conditions controlled the yield of bioactive compounds and the antioxidant activity of C. chinensis Franch, which can provide important information for the industrial extraction.

Keywords:Coptis chinensis Franch;Antioxidant capacity;Bioactive compounds;Optimal extraction

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Do QD, Angkawijaya AE, Nguyen PLT, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH, J. Food Drug Anal., 22, 296 (2014)
Sultana B, Anwar F, Ashraf M, Molecules, 14, 2167 (2009)
Peng J, Han X, Xu Y, Qi Y, Xu L, Xu Q, J. Liq. Chromatogr. Relat. Technol., 30, 2929 (2007)
Bravo L, Nutr. Rev., 56, 317 (1998)
Auyeung KKW, Ko JKS, Int. J. Mol. Med., 24, 571 (2009)
Deighton N, Brennan R, Finn C, Davies HV, J. Sci. Food Agric., 80, 1307 (2000)
Schinella G, Tournier H, Prieto J, De Buschiazzo PM, Rios J, Life Sci., 70, 1023 (2002)
Jang MH, Kim HY, Kang KS, Yokozawa T, Park JH, Arch. Pharm. Res., 32, 341 (2009)

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[1] Hayyan M, Hashim MA, AlNashef IM, Chem. Rev., 116(5), 3029 (2016)

[2] Devasagayam T, Tilak J, Boloor K, Sane KS, Ghaskadbi SS, Lele R, J. Assoc. Physicians India, 52, 794 (2004)

[3] Nordberg J, Arner ES, Free Radical Biol. Med., 31, 1287 (2001)

[4] Xuan SH, Park YM, Ha JH, Jeong YJ, Park SN, Pharmacol. Rep., 69, 1224 (2017)

[5] Shirwaikar A, Shirwaikar A, Rajendran K, Punitha ISR, Biol. Pharm. Bull., 29, 1906 (2006)

[6] Liu B, Li W, Chang Y, Dong W, Ni L, J. Pharm. Biomed. Anal., 41, 1056 (2006)

[7] Yan D, Jin C, Xiao XH, Dong XP, J. Biochem. Biophys. Methods, 70, 845 (2008)

[8] Lee DU, Kang YJ, Park MK, Lee YS, Seo HG, Kim TS, Kim CH, Chang KC, Life Sci., 73, 1401 (2003)

[9] Kong W, Wang J, Xiao X, Chen S, Yang M, Analyst, 137, 216 (2012)

[10] Ding D, Pharmacodynamic Action an Clinic of Chinese Medicinal Materials, 54-56, China Medicine Technology Press, Beijing, China (1999).

[11] Teng H, Choi YH, Food Chem., 142, 299 (2014)

[12] Lotito SB, Frei B, Free Radical Biol. Med., 41, 1727 (2006)

[13] Schinella GR, Tournier HA, Prieto JM, de Buschiazzo PM, Rios JL, Life Sci., 70, 1023 (2002)

[14] Xu P, Gao Y, Zhang X, Zhang L, Xu K, rhizoma coptidis, Lishizhen Med. Mater. Med. Res., 18, 3079-3080 (2007).

[15] Barroso MF, Noronha J, Delerue-Matos C, Oliveira M, J. Agric. Food Chem., 59, 5062 (2011)

[16] Chandler S, Dodds J, Plant Cell Rep., 2, 205 (1983)

[17] Shetty K, Curtis OF, Levin RE, Witkowsky R, Ang W, J. Plant Physiol., 147, 447 (1995)

[18] Costa AS, Alves RC, Vinha AF, Barreira SV, Nunes MA, Cunha LM, Oliveira MBP, Ind. Crop. Prod., 53, 350 (2014)

[19] Benzie IF, Strain JJ, Anal. Biochem., 239, 70 (1996)

[20] Pinelo M, Rubilar M, Jerez M, Sineiro J, Nunez MJ, J. Agric. Food Chem., 53, 2111 (2005)

[21] Do QD, Angkawijaya AE, Nguyen PLT, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH, J. Food Drug Anal., 22, 296 (2014)

[22] Sultana B, Anwar F, Ashraf M, Molecules, 14, 2167 (2009)

[23] Peng J, Han X, Xu Y, Qi Y, Xu L, Xu Q, J. Liq. Chromatogr. Relat. Technol., 30, 2929 (2007)

[24] Bravo L, Nutr. Rev., 56, 317 (1998)

[25] Auyeung KKW, Ko JKS, Int. J. Mol. Med., 24, 571 (2009)

[26] Deighton N, Brennan R, Finn C, Davies HV, J. Sci. Food Agric., 80, 1307 (2000)

[27] Schinella G, Tournier H, Prieto J, De Buschiazzo PM, Rios J, Life Sci., 70, 1023 (2002)

[28] Jang MH, Kim HY, Kang KS, Yokozawa T, Park JH, Arch. Pharm. Res., 32, 341 (2009)