골유도재생을 위한 젤란검과 글리콜 키토산 조성 흡수성 차폐막의 평가

장진운; 김 진; 국민석; 이기영; Jin-Woon Jang; Jin Kim; Min-Suk Kook; Ki-Young Lee

Polymer(Korea), Vol.42, No.5, 874-881, September, 2018

DOI10.7317/pk.2018.42.5.874 Export Citation

골유도재생을 위한 젤란검과 글리콜 키토산 조성 흡수성 차폐막의 평가

Evaluation of Gellan Gum/Glycol Chitosan Bioabsorbable Membrane for Guided Bone Regeneration

장진운, 김 진¹, 국민석², 이기영^{3, †}

전남대학교 신화학소재공학과
¹헬스케어의공학연구소
²전남대학교 치의학전문대학원 구강악안면외과학교실
³㈜바이오코스모스, 전남대학교 화학공학부

Jin-Woon Jang, Jin Kim¹, Min-Suk Kook², and Ki-Young Lee^{3, †}

Department of Advanced Chemicals and Engineering, Chonnam National University, Gwangju 61186, Korea
¹Research Center for Healthcare-Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea
²Department of Oral and Maxillofacial Surgery, Chonnam National University School of Dentistry, Gwangju 61186, Korea
³School of Chemical Engineering and Biocosmos Co., Chonnam National University, Gwangju 61186, Korea

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초록

골유도재생을 위해 젤란검(GEL)과 글리콜 키토산(GC) 조성막을 완충생리식염수(PBS)로 가교시켜 흡수성 차폐막(GBR)을 제조하였다. GEL/GC 조성막의 특성 분석을 위해 푸리에 변환 적외선 분광학, 분해도, 접촉각, 인장강도, 세포생존율을 평가하였다. 결과적으로 GEL 조성막에 GC의 함량이 증가할수록 막의 분해율 및 물리적 특성이 감소하였다. 랫 두개골에 결손부를 형성하여 흡수성 차폐막을 고정시켜 마이크로 CT, 조직 학적 평가를 통해 신생골을 관찰하였다. 신생골 재생의 양은 GEL/GC 군이 대조군보다 유의하게 더 컸다. GEL/GC 흡수성 차폐막은 연조직을 차단하고 새로운 골형성에 효과가 있는 것을 확인하였다.

Bioabsorbable membrane was prepared using gellan gum (GEL)-glycol chitosan (GC) with phosphate-buffered saline (PBS) as a crosslinker for guided bone regeneration (GBR). The GEL/GC membrane was characterized by Fourier transform infrared (FTIR), degradation, contact angle, tensile strength and cell viability. The result showed that by increasing the GC content in GEL membrane, the degradable and mechanical properties were decreased. New bone was observed through micro-CT and histological evaluation of forming a defect in rat calvarial and fixing the membrane. The amount of newbone regeneration was significantly greater in GEL/GC group than the control. The GEL/GC membrane blocked the soft tissue and has effects on the new bone formation.

Keywords:gellan gum;glycol chitosan;membrane;guided bone regeneration

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[Referenced By]

[1] Yoshiya U, Kunio I, Takamitsu M, Takahiro K, Jitoshi N, Kazuomi S, Kazuo R, Biomaterials, 23, 2027 (2002)

[2] Lee SH, Lim YM, Jeong SI, An SJ, Kang SS, Jeong CM, Huh JB, J. Adv. Prosthodont., 7, 485 (2015)

[3] Xie Y, Park JS, Kang SK, J. Korea Acad. Industr. Coop. Soc., 16, 6403 (2015)

[4] Lee JH, You H, Kim YK, Shin HK, Lim HC, Kim YT, Lee JS, Jung UW, Choi SH, Implantology, 19, 17 (2015)

[5] Agrawal CM, Niederauer GG, Athanasiou KA, Tissue Eng., 1, 241 (1995)

[6] Zubery Y, Goldlust A, Alves A, Nir E, J. Periodontol., 78, 112 (2007)

[7] Zisch AH, Zeisberger SM, Ehrbar M, Djonov V, Weber CC, Ziemiecki A, Pasquale EB, Hubbell JA, Biomaterials, 25, 3245 (2004)

[8] Dahlin C, Linde A, Gottlow J, Nyman S, Plats. Reconstr. Surg., 81, 672 (1988)

[9] Pereira DR, Canadas RF, Correia JS, Marques AP, Reis RL, Oliveira JM, Key Eng. Mater., 587, 255 (2014)

[10] Matricardi P, Cencetti C, Ria R, Alhaique F, Coviello T, Molecules, 14, 3376 (2009)

[11] Park HW, Kim HY, Kwon SY, Khang G, Kim YS, Polym. Korea, 39, 145 (2014)

[12] You HS, Cho MO, Cho IS, Li Z, Kim NH, Jang MK, Kang SW, Huh KM, Polym. Korea, 40, 329 (2016)

[13] Jang MK, Nah JW, J. Korean Ind. Eng. Chem., 19(5), 457 (2008)

[14] Kulkarni RV, Mangond BS, Mutalik S, Sa B, Carbohydr. Polym., 83, 1003 (2011)

[15] Kim J, Cheon SY, Kook MS, Lee CM, Lee KY, Polym. Korea, 42(2), 280 (2018)

[16] Ismail NA, Mohamad SF, Ibrahim MA, Amin KAM, Adv. Biomat., 2014, 1 (2014)

[17] Singh R, Mahesh L, Shukla S, Int. J. Oral. Implantol. Clin. Res., 4, 68 (2013)

[18] Li X, Xie H, Lin J, Xie W, Ma X, Polym. Degrad. Stabil., 94, 1 (2009)

[19] Bhuvaneshwari S, Sruthi D, Sivasubramanian V, Kalyani N, Sugunabai J, IJERA, 1, 292 (2007)

[20] Liu L, Wang B, Gao Y, Bai TC, Carbohydr. Polym., 97, 155 (2013)

[21] Fisher PD, Venugopal G, Milbrandt TA, Hilt JZ, Puleo DA, J. Biomed. Mater. Res. A, 103, 2365 (2015)

[22] Ko HA, Jang JE, Kim HS, Park CH, Kwon SY, Chung JW, Khang GS, Polymer, 38, 166 (2013)

[23] Park JK, Choi CY, Nam JP, Park SC, Park YH, Jang MK, Nah JW, Polymer, 38, 85 (2014)

[24] Chang SJ, Kuo SM, Liu WT, Niu CCG, Lee MW, Wu CS, J. Med. Biol. Eng., 30, 99 (2010)

[25] You R, Lv H, Xiao Y, Yang D, Su Z, Yan C, Curr. Signal Transduct. Ther., 11, 28 (2016)

[26] Klokkevold PR, Vandemark L, Kenney EB, Bernard GW, J. Periodontol., 67, 1170 (1996)

[27] Kim KH, Park YJ, Park JW, Lee YC, Cho BO, Ahn BK, J. Kor. Oral Maxillofac. Surg., 28, 284 (2002)

[28] Chen XF, Liu Y, Miao LY, Wang YY, Ren SS, Yang XB, Hu Y, Sun WB, Int. J. Nanomed., 11, 3154 (2016)

[29] You H, Lee EU, Kim YK, Kim BC, Park JY, Lim HC, Lee JS, Noh IS, Jung UW, Choi SH, Biomaterials, 18, 4 (2014)