Electrosprayed Folic Acid-Conjugated Ursolic Acid Nanoparticles for Tumor Therapy

Guk Young Ahn; Dong-Hyun Paik; Ki-Young Jeong; Seung-Woon Baek; Rae-Hyoung Kang; Eun Seong Lee; Sung-Wook Choi

Macromolecular Research, Vol.26, No.7, 573-576, July, 2018

DOI10.1007/s13233-018-6089-x Export Citation

Electrosprayed Folic Acid-Conjugated Ursolic Acid Nanoparticles for Tumor Therapy

Guk Young Ahn, Dong-Hyun Paik, Ki-Young Jeong, Seung-Woon Baek, Rae-Hyoung Kang, Eun Seong Lee, and Sung-Wook Choi^†

Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi 14662, Korea

E-mail:

We describe a simple method for the fabrication of folic acid-conjugated ursolic acid (FA-UA) nanoparticles using electrospraying. The FA-UA nanoparticles had an average size of 90.6 nm and exhibited greater cellular uptake compared with pristine UA and electrosprayed UA nanoparticles. We also evaluated the cytotoxicity of pristine UA, electrosprayed UA nanoparticles, and FA-UA nanoparticles over time and under different UA concentrations. In vitro, the FA-UA nanoparticles showed significantly higher cytotoxicity in KB cells compared with pristine UA and electrosprayed UA nanoparticles. These results were corroborated by LIVE/DEAD staining and fluorescence imaging of cancer cells. Thus, FA-UA nanoparticles can potentially be used for targeting drugs in cancer therapies.

Keywords:folic acid;ursolic acid;electrospraying;tumor therapy

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[1] Dwivedi GR, Maurya A, Yadav DK, Khan F, Darokar MP, Srivastava SK, Chem. Biol. Drug Des., 86, 272 (2015)

[2] Liu J, J. Ethnopharmacol., 49, 57 (1995)

[3] Janicsak G, Veres K, Kallai M, Mathe I, Chromatographia, 58, 295 (2003)

[4] Ikeda Y, Murakami A, Ohigashi H, Mol. Nutr. Food Res., 52, 26 (2008)

[5] Baricevic D, Sosa S, Loggia RD, Tubaro A, Simonovska B, Krasna A, Zupancic A, J. Ethnopharmacol., 75, 125 (2001)

[6] Hsu HY, Yang JJ, Lin CC, Cancer Lett., 111, 7 (1997)

[7] Yan SL, Huang CY, Wu ST, Yin MC, Toxicol. Vitro, 24, 842 (2010)

[8] Huang CY, Lin CY, Tsai CW, Yin MC, Toxicol. Vitro, 25, 1274 (2011)

[9] Bonaccorsi I, Altieri F, Sciamanna I, Oricchio E, Grillo C, Contartese G, Galati EM, Cancer Lett., 263, 130 (2008)

[10] Kondo M, MacKinnon SL, Craft CC, Matchett MD, Hurta RA, Neto CC, J. Sci. Food Agric., 91, 789 (2011)

[11] Hedges AR, Chem. Rev., 98(5), 2035 (1998)

[12] Zhang W, Hong D, Zhou Y, Zhang Y, Shen Q, Li JY, Hu LH, Li J, Biochim. Biophys. Acta, 1760, 1505 (2006)

[13] Zhou XJ, Hu XM, Yi YM, Wan J, Drug Dev. Ind. Pharm., 35, 305 (2009)

[14] Zhang H, Li X, Ding J, Xu H, Dai X, Hou Z, Zhang K, Sun K, Sun W, Int. J. Pharm., 441, 261 (2013)

[15] de Araujo Lopes SC, Novais MVM, Teixeira CS, Honorato-Sampaio K, Pereira MT, Ferreira LA, Braga FC, Oliveira MC, Biomed. Res. Int., 2013, 467147 (2013)

[16] Brannon-Peppas L, Blanchette JO, Adv. Drug Deliv. Rev., 56, 1649 (2004)

[17] Elbayoumi TA, Torchilin VP, Int. J. Pharm., 357, 272 (2008)

[18] Yang G, Yang T, Zhang W, Lu M, Ma X, Xiang G, J. Agric. Food Chem., 62, 2207 (2014)

[19] Gao Y, Li Z, Xie X, Wang C, You J, Mo F, Jin B, Chen J, Shao J, Chen H, Jia L, Eur. J. Pharm. Sci., 70, 55 (2015)

[20] Sridhar R, Ramakrishna S, Biomatter, 3, e24281 (2013)

[21] Nguyen DN, Clasen C, den Mooter GV, J. Pharm. Sci., 105, 2601 (2016)

[22] Ai J, Xu Y, Li D, Liu Z, Wang E, Talanta, 101, 32 (2012)

[23] Zhang Z, Jia J, Lai Y, Ma Y, Weng J, Sun L, Bioorg. Med. Chem., 18, 5528 (2010)

[24] Song H, Su C, Cui W, Zhu B, Liu L, Chen Z, Zhao L, Biomed. Res. Int., 2013, 723158 (2013)

[25] Liu F, Deng D, Chen X, Qian Z, Achilefu S, Gu Y, Mol. Imaging Biol., 12, 595 (2010)

[26] Kashiwada Y, Nagao T, Hashimoto A, Ikeshiro Y, Okabe H, Cosentino LM, Lee KH, J. Nat. Prod., 63, 1619 (2000)

[27] Pillai JJ, Thulasidasan AKT, Anto RJ, Devika NC, Ashwanikumar N, Kumar GV, RSC Adv., 5, 25518 (2015)

[28] Bock N, Woodruff MA, Steck R, Hutmacher DW, Farrugia BL, Dargaville TR, Macromol. Biosci., 14, 202 (2014)

[29] Jaworek A, Krupa A, Lackowski M, Sobczyk AT, Czech T, Ramakrishna S, Sundarrajan S, Pliszka D, Fibers Text. East. Eur., 17, 77 (2009)

[30] Paik DH, Choi SW, Macromol. Rapid Commun., 35(11), 1033 (2014)

[31] Alkilany AM, Murphy CJ, J. Nanopart. Res., 12, 2313 (2010)

[32] Ryu TK, Lee GJ, Rhee CK, Choi SW, Macromol. Biosci., 15, 1469 (2015)

[33] Raju HB, Hu Y, Vedula A, Dubovy SR, Goldberg JL, PLoS One, 6, e17452 (2011)

[34] Li J, Guo WJ, Yang QY, World J. Gastroenterol., 8, 493 (2002)