In recent years, molecular-sieve graphene oxide (GO) membranes have shown a great potential to realize high flux and high-selectivity mixture separation, at low energy cost. Hence, in this study, GO nanocomposite membranes were prepared for hydrogen separation. To this purpose, the modified tubular gamma-alumina supports were prepared by coating boelmiite sol on a-alumina support. Then, the GO top layer was coated on the modified gamma-alumina support using GO colloidal sol prepared by modified hummer method. Consequently, effects of various concentrations of dip-coating sol were evaluated on GO graphene membrane performance, in terms of hydrogen separation at various pressure gradient and temperatures. Regarding to characterization analyses, uniform GO layer formation was confirmed on modified support. As a result of gas permeance test of GO nanocomposite membrane, pressure gradient effect was positive on membrane performance, while negative effect on H-2/CO2 and H-2/N-2, selectivities was indicated versus lowest concentration of dip coating sol. On the other, far all cases, negative effect was observed for hydrogen selectivity versus temperature. In particular, for the best GO membrane sample, at room temperature and 3 bar pressure gradient, H-2/CO2 and H-2/N-2 values were obtained 38.5 and 16.5 (H-2 permeance equal 5.9*10(-7) mol/m(2) s Pa), respectively, while these parameters values at 473 K and 1 bar pressure gradient were 15.7, 10.6 (H-2 permeance equal 7.6 * 10(-7) mol/m(2) s Pa). Moreover, regarding to effects of dip-coating sol concentration, the best choice was introduced sample 1 (with highest concentration of dip coating sol) from performance viewpoint.