We report a one-step green synthesis of multifunctional silver nanoparticles AgNPs (Ag/AgCl NPs) using aqueous stem extracts of D. trifoliata and S. alba. Optimization results revealed that the maximum rate of synthesis could be achieved with 1.5 x 10(-3) M and 2 x 10(-3) m AgNO3 solution at room temperature in 4 h and 2 h respectively. The synthesized AgNPs were characterized by UV-vis spectroscopy, the bands were observed at 445 nm and 450 nm. High resolution transmission electron micrographs (HR-TEM) showed mostly spherical particles having diameters ranging from 20 to 60 nm. Energy dispersive x-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) confirmed elemental silver and chlorine, X-Ray diffraction (XRD) revealed the formation of face centered cubic. Interaction between human serum albumin (HSA) and AgNPs were studied at different temperatures. The Stern Volmer quenching constant, binding constant, number of binding sites thermodynamic parameters were calculated. Thermodynamic parameters (Delta G(0), Delta H-0& Delta S-0) hint that the binding process occurs spontaneously. Circular dichroism indicates that a helicity of HSA decreases due to the interaction with AgNPs. AgNPs showed potent antioxidant and anti-inflammatory activity. Our results reveal promising approach for alternative nano drug development.