A new system for ultra-trace detection of imipramine (IMI) in urine and whole blood samples was designed by coupling fast Fourier transformation square wave voltammetry (FFTSWV) and electromembrane extraction (EME). This is the first report of the modification carbon paste electrode (CPE) through the Sr(VO3)(2) nanoparticles dopedwith phytic acid for the FFTSWV determination of IMI. The extraction of IMI molecules from the solution of phosphate buffer was performed by using 2-Nitrophenyl octyl ether as the supported liquid membrane in the pores of the hallow fiber (HF). The optimal important parameters for the EME-FFTSWV response were determined as follow: frequency: 5670 Hz, amplitude: 30 mV, pH of the sample solution: 4.5, pH of the acceptor solution: 4; EME time: 15 min; EME potential: 150 V. According to the calibration curves plotted for urine and whole blood samples, two wide linear ranges of 0.02-1000 and 0.2-1000 ng/mL with determination coefficient of 0.982 and 0.990 were obtained. The LODs of 0.001 and 0.01 ng mL(-1), and also LOQs of 0.02 and 0.2 ng mL(-1), were calculated for urine and whole blood, respectively. In addition, relative standard deviation (RSD) within the range of (4.3-5.5) and (5.2-6.1) were achieved in urine and whole blood samples. (C) 2018 The Electrochemical Society.