Bimetallic nanomaterials have potential catalytic behaviour in hydrogenation, clean-energy production, catalysis and sensors due to their great stability, loftier activity unique electrical and chemical properties. Herein, we prepared Pd-Ag bimetallic nanoparticles synthesized by using fungal extracted aqueous method, which is environmentally friendly cost-effective and simple procedure. The fabricated Pd-Ag bimetallic nanoparticles were investigated by small area electron diffraction (SAED), transmission electron microscopy (TEM) X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectroscopy (EDX) analysis. The electrochemical response of uric acid (UA) at Pd-Ag/CPE was studied in 0.1 M phosphate buffer solution at various pH, concentration and scan rate was investigated. Compare to Bare CPE, the Pd-Ag nanocomposite modified electrode displayed the highest electrocatalytic activity for the detection of UA A linear response in the range of 4.69-273 nM with remarkable detection limit of 5.543 nM (C-DL = 3 sigma/M) and quantification limit of 16.64 nM (C-QL = 10 sigma/M) was obtained. The established nanoparticles (Pd-Ag) embedded with carbon paste electrode (Pd-Ag/CPE) makes a good analytical tool for the sensing of UA in the biological and pharmaceutical samples.