This study investigates the interfacial behavior and heat transfer mechanisms associated with flow boiling of R-134a in a micro-channel test module. The test module features 100 of 1 x 1 mm(2) square microchannels. Large length of micro-channels used (609.6 mm) is especially important to capturing broad axial variations of both flow and heat transfer behavior. The fluid is supplied to the test module in subcooled state to enable assessment of both the subcooled boiling and saturated boiling regions. The study employs a combination of temperature measurements along the test module and high-speed video to explore crucial details of the flow, including dominant flow regimes, flow instabilities, and downstream dryout effects. It is shown that, unlike macro-channel flows, where flow regimes can be clearly demarcated, flow regimes in micro-channels are associated with transient fluctuations that are induced by flow instabilities. The dominant flow behavior and associated dryout effects are characterized with the aid of a new transient flow regime map and a dryout map, respectively. Two sub-regions of the subcooled boiling region, partially developed boiling (PDB) and fully developed boiling (FDB), are examined relative to dominant interfacial and heat transfer mechanisms, and a previous correlation is identified for accurate prediction of the heat transfer coefficient for both PDB and FDB. The saturated boiling region is shown to consist of three separate sub-regions: nucleate boiling dominated for qualities below 0.3, combined nucleate and convective boiling for qualities between 0.3 and 0.5, and convective boiling dominated for qualities above 0.5. Above 0.5, dryout effects begin to take effect, causing a gradual decline in the heat transfer coefficient followed downstream by a more severe decline. A previous correlation is identified for prediction of the heat transfer coefficient in the saturated boiling region. (C) 2018 Elsevier Ltd. All rights reserved.