Gaseous fuels can be used in diesel engines to improve combustion and obtain more favorable emission. Vibration and noise formation in diesel engines is a rather complex phenomenon which is created during combustion of fuels and leads to a reduction in vehicle comfort. Although there are studies in the literature that examine the noise and vibration of the diesel engine using different biofuels, there is no study that thoroughly examines the effect of combined utilization of Foeniculum vulgare oil biodiesel (FVB) and hydroxy gas (HHO) on vibration, noise and combustion characteristics. Therefore, this study aimed to explore the effects of FVB, a promising biodiesel feedstock, and HHO dual fuel operation on vibration, noise and exhaust emissions of a diesel engine. The vibration, noise and emission data obtained by the use of diesel fuel were taken as a reference and the effects of FVB and HHO mixture utilization on vibration, noise and emission formation were examined. The results show that the total vibration and noise generated by the engine was decreased by the use of FVB. In addition, the utilization of HHO together with biodiesel further reduced the engine vibration and noise according to experimental data. According to exhaust emission formation measurements, the minimum carbon monoxide values were obtained when the engine was fueled with HHO and FVB mixtures. However, CO2 and NOX emissions increased with the combination of HHO and FVB usage.