A series of CuO doped V2O5-WO3/TiO2 based commercial selective catalytic reduction (SCR) catalysts were synthesized via the improved impregnation method for simultaneous NO removal and Hg-0 oxidation under simulated coal-fired flue gas at a temperature range of 150-400 degrees C. Several characterization techniques, including Brunauer-Emmett-Teller analysis, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and temperature-programmed reduction of H-2 (H-2-TPR), were used to characterize the catalysts. The results indicated that Cu-3-SCR catalyst exhibited the superior catalytic activity and a wide active temperature window for simultaneous NO removal and Hg-0 oxidation. The effects of flue gas components on the catalytic activity were also investigated. The results indicated that Cu-3-SCR catalyst showed good performances on SO2 tolerance and H2O resistance. The effect of He on NO removal was almost negligible. However, the copresence of NO and NH3 obviously inhibited the Hg-0 oxidation activity. Further study revealed that this inhibiting effect was weakened as the consumption of NH3. The BET and XRD results suggested that the highly dispersed Cu species was beneficial to the superior catalytic activity of the Cu3-SCR catalyst. The XPS and H-2-TPR analyses indicated that the Cu-3-SCR catalyst possessed abundant chemisorbed oxygen and good redox ability, which was ascribed to the strong synergy between CuO and V2O5 on the catalyst. The redox cycle of V4+ + Cu2+ <-> V5+ + Cu+ in Cu-3-SCR catalyst significantly improved the catalytic activity for simultaneous NO removal and He oxidation. The mechanism of Hg-0 oxidation over the Cu-3-SCR catalyst was also investigated.