A novel bacterial strain MAH-10(T) was isolated from soil sample of a Chinese cabbage garden, Republic of Korea and was characterized using a polyphasic approach. Cells were Gram-staining negative, rod-shaped, yellowish orange colored, and motile. The strain was aerobic, catalase and oxidase are positive, and optimum growth temperature and pH were 28 A degrees C and 6.5, respectively. Flexirubin-type pigments were found to be present. On the basis of 16S rRNA gene sequence analysis, strain MAH-10(T) belongs to the genus Flavobacterium and is most closely related to Flavobacterium tyrosinilyticum KCTC 42726(T) (98.7%). On the basis of phylogenetic tree, other closely related species are Flavobacterium banpakuense KACC 14225(T) (98.3%) and Flavobacterium chungbukense KACC 15048(T) (97.6%). In DNA-DNA hybridization tests, the DNA relatedness between strain MAH-10(T) and its closest phylogenetic neighbor was below 45.0%. The DNA G+C content was 37.2 mol% and the predominant respiratory quinone was menaquinone-6. The major cellular fatty acids were C-15:0 iso, C-16:0, and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c). On the basis of DNA-DNA hybridization results and genotypic, chemotaxonomic, and physiological data analysis, it is demonstrated that strain MAH-10(T) represented a novel species within the genus Flavobacterium, for which the name Flavobacterium chungangensis is proposed. The type strain is MAH-10(T) (=KACC 19296(T)=CGMCC 1.16226(T)). The NCBI GenBank accession number for the 16S rRNA gene sequence of strain MAH-10(T) is KY964277 and the digital protologue database (DPD) Taxon Number of strain MAH-10(T) is TA00296.