A promising concept of aqueous two-phase extraction (ATPE) is the Tunable Aqueous Polymer Phase Impregnated Resins (TAPPIR (R)) technology. Thereby, one phase of an aqueous two-phase system (ATPS) is immobilized inside porous solids, which are then dispersed in the second aqueous phase. Beside extraction performance, the reusability of the impregnated solids for several cycles decides upon the competitiveness of TAPPIR (R). Multistage extraction experiments using a polyethylene glycol 4000/citrate ATPS to partition lysozyme were investigated for TAPPIR (R) and ATPE. A stable impregnation was demonstrated for multiple cycles. Moreover, it was found that multistage extraction processes should not be designed on basis of the first extraction as the yield changes in the following stages.