This work studied the optimal conditions for pyrolysis of Prosopis juliflora wood in fixed-bed tubular reactor. The optimal conditions are measured by performing pyrolysis experiment with respect to wood properties such as particle size, moisture and pyrolysis condition such as, temperatures, heating rates. Higher solid yield (36.8%) was recorded for a slower heating rate of larger particle size at lower temperatures. Further, higher liquid yield (38.3%) was observed while maintaining high heating rate and temperature. It is observed that with increase in particle size, the yield of char and gas decreases and bio-oil increases. The literature reported biomass pyrolysis kinetic model is validated for Prosopis juliflora wood. The kinetic models are able to predict the performance of fixed-bed tubular reactor in terms of pyrolysis product properties. The validated kinetic model may be used for the design of commercial fixed bed pyrolysis reactor to process Prosopis juliflora wood.