In this present study, spherical AgNPs have been synthesized by chemical reduction method in the presence of different surfactants i.e. trisodium-citrate and polyvinylpyrrolidone. Further, these synthesised AgNPs have been functionalized with different concentrations of glycopeptide antibiotic (vancomycin). The interaction between AgNPs and antibiotic has been studied using various analytical techniques i.e. UV-vis absorption spectroscopy, Dynamic light scattering, and X-ray diffraction. Further, the nature of bonding between antibiotic and AgNPs has been probed by Fourier transform infrared spectroscopy, which shows the amine bonding between vancomycin and silver nanoparticles surface. The role of different nature of surfactants on attachment and interaction mechanism of drug with AgNPs has been studied. (C) 2017 Elsevier B.V. All rights reserved.