In the present study, hexagonal mesoporous silica (HMS) was synthesized and modified by tannic acid as a natural poly-phenol and amine (TA-A-HMS) and was applied for the adsorption of bovine serum albumin (BSA) from aqueous media. To investigate the structure of HMS and TA-A-HMS, SEM, TEM, XRD, BET and FTIR analysis were applied. The effects of pH, adsorbent dosage, contact time and temperature on the BSA adsorption were studied. After modification, BET surface area of HMS was reduced from 885 m(2)/g to 51 m(2)/g which confirms the presence of tannin and amine groups that inhibit the adsorption of nitrogen molecules. According to the results of equilibrium data, it is shown that Langmuir isotherm with maximum adsorption capacity of 1000 mg/g is the predominant model and adsorption is mono-layer. Kinetic and thermodynamic studies also reveal that adsorption kinetic followed by pseudo-second order model and the adsorption process is exothermic. (C) 2018 The Society of Powder Technology Japan. Published by Elsevier B.V. and The Society of Powder Technology Japan. All rights reserved.