Self-assembly of tumor-targeting RNA nanoball for carrier-free delivery of therapeutic RNA

Sangwoo Han; Jong Bum Lee

Journal of Industrial and Engineering Chemistry, Vol.64, 90-96, August, 2018

DOI10.1016/j.jiec.2018.03.020 Export Citation

Self-assembly of tumor-targeting RNA nanoball for carrier-free delivery of therapeutic RNA

Sangwoo Han, and Jong Bum Lee^†

Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemungu, Seoul, 02504, Republic of Korea

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Rolling circle transcription (RCT) has been studied for enhancing the delivery efficiency of therapeutic RNA by densely packing RNA strands with particle formation. This facile fabrication method has been successfully employed to generate RNA nanoballs containing functional short interference RNA. In addition, the nanoballs produced by RCT showed high stability from the nuclease degradation. With these advantages, using biotin-labeled ribonucleoside triphosphate (rNTP), target specific and non toxic bubble RNA nanoball (BNB) was synthesized. To achieve tumor-targeting, epidermal growth factor receptor antibody (EGFR Ab) was used to target the tumor cells by labeling the target ligands with biotin. avidin interaction. Target gene silencing by EGFR Ab-labeled RNA nanoball was confirmed in vitro. Therefore, RNA nanoball can be utilized for tumor cell-targeting delivery of therapeutic RNA.

Keywords:Nucleic acid;Rolling circle transcription (RCT);Anti-epidermal growth factor receptors;antibody (anti-EGFR Ab);siRNA;RNA nanoball

[References]

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Kota J, Chivukula RR, O’Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Cell, 137, 1005 (2009)
Wu Y, Crawford M, Yu B, Mao Y, Nana-Sinkam SP, Lee LJ, Mol. Pharm., 8, 1381 (2011)
Wang P, Zhang L, Xie Y, Wang N, Tang R, Zheng W, Jiang X, Adv. Sci., 1700175 (2017)
Ha JS, Lee JS, Jeong J, Kim H, Byun J, Kim SA, Lee HJ, Chung HS, Lee JB, Ahn DR, J. Control. Release, 250, 27 (2017)
Sun W, Ji W, Hall JM, Hu Q, Wang C, Beisel CL, Gu Z, Angew. Chem.-Int. Edit., 54, 12029 (2015)
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Roh YH, Deng JZ, Dreaden EC, Park JH, Yun DS, Shopsowitz KE, Hammond PT, Angew. Chem.-Int. Edit., 55, 3347 (2016)
Kim H, Jeong J, Kim D, Kwak G, Kim SH, Lee JB, Adv. Sci., 1600523 (2017)
Zhu GZ, Hu R, Zhao ZL, Chen Z, Zhang XB, Tan WH, J. Am. Chem. Soc., 135(44), 16438 (2013)
Hu R, Zhang X, Zhu G, Chen T, Fu T, Tan W, Angew. Chem.-Int. Edit., 126, 5931 (2014)
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[Referenced By]

[1] Kim H, Park Y, Kim J, Jeong J, Han S, Lee JS, Lee JB, ACS Comb. Sci., 18, 87 (2016)

[2] Grabow WW, Jaeger L, Accounts Chem. Res., 47, 1871 (2014)

[3] Guo PX, Nat. Nanotechnol., 5(12), 833 (2010)

[4] Liu X, Zhou J, Yu T, Chen C, Cheng Q, Sengupta K, Huang Y, Li H, Liu C, Wang Y, Angew. Chem.-Int. Edit., 53, 11822 (2014)

[5] Lee JB, Hong J, Bonner DK, Poon Z, Hammond PT, Nat. Mater., 11(4), 316 (2012)

[6] Brunner K, Harder J, Halbach T, Willibald J, Spada F, Gnerlich F, Sparrer K, Beil A, Mockl L, Brauchle C, Angew. Chem.-Int. Edit., 54, 1946 (2015)

[7] Kota J, Chivukula RR, O’Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Cell, 137, 1005 (2009)

[8] Wu Y, Crawford M, Yu B, Mao Y, Nana-Sinkam SP, Lee LJ, Mol. Pharm., 8, 1381 (2011)

[9] Wang P, Zhang L, Xie Y, Wang N, Tang R, Zheng W, Jiang X, Adv. Sci., 1700175 (2017)

[10] Ha JS, Lee JS, Jeong J, Kim H, Byun J, Kim SA, Lee HJ, Chung HS, Lee JB, Ahn DR, J. Control. Release, 250, 27 (2017)

[11] Sun W, Ji W, Hall JM, Hu Q, Wang C, Beisel CL, Gu Z, Angew. Chem.-Int. Edit., 54, 12029 (2015)

[12] Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science, 274(5295), 2065 (1996)

[13] Shopsowitz KE, Roh YH, Deng ZJ, Morton SW, Hammond PT, Small, 10, 1623 (2014)

[14] Keller M, J. Control. Release, 103, 537 (2005)

[15] Werth S, Urban-Klein B, Dai L, Hobel S, Grzelinski M, Bakowsky U, Czubayko F, Aigner A, J. Control. Release, 112, 257 (2006)

[16] Park Y, Kim H, Lee JB, ACS. Biomater. Sci. Eng., 2, 616 (2016)

[17] Roh YH, Deng JZ, Dreaden EC, Park JH, Yun DS, Shopsowitz KE, Hammond PT, Angew. Chem.-Int. Edit., 55, 3347 (2016)

[18] Kim H, Jeong J, Kim D, Kwak G, Kim SH, Lee JB, Adv. Sci., 1600523 (2017)

[19] Zhu GZ, Hu R, Zhao ZL, Chen Z, Zhang XB, Tan WH, J. Am. Chem. Soc., 135(44), 16438 (2013)

[20] Hu R, Zhang X, Zhu G, Chen T, Fu T, Tan W, Angew. Chem.-Int. Edit., 126, 5931 (2014)

[21] Yang L, Mao H, Wang YA, Cao Z, Peng X, Wang X, Duan H, Ni C, Yuan Q, Adams G, Small, 5, 235 (2009)