In many places around the world, the fluoride concentration in ground water as raw water is higher than the threshold standard for healthy drinking water. The aim of this work is to study the characteristics of an anionic membrane that will be the subject of an application for defluoridation by electrodialysis. The first objective of this work is to evaluate the physicochemical characteristics of the membrane used in experiments by determination of the exchange capacity, water uptake, ionic conductivity, and transport number (Hittorf method). The second part consists of studying the effect of different parameters such as electrodialysis time, supporting electrolyte concentration, coexisting ions (such as SO42-, NO3- and Cl-), current density, and the pH of the solution to evaluate the effectiveness of the electrodialysis defluoridation process. The high percentage of defluoridation obtained after treatment of industrial effluent (80.3%) reflects the good efficiency of the electrodialysis process.