Extraction efficiency and physiochemical properties of acid and pepsin soluble collagens from the skin of golden carp (Probarbus pillion as influenced by ultrasonication were studied. The ultrasound treatment (20 kHz) with increasing amplitudes from 20 to 80% increased the yield of acid soluble collagen (ASC) (P < 0.05). When the ultrasound at an amplitude of 80% was employed in combination with pepsin (0.1. 0.5 and 1%), the marked increase in pepsin soluble collagen (PSC) was obtained when pepsin level increased (P < 0.05). The yield of ASC and PSC increased as ultrasonication time increased (P < 0.05). ASC and PSC with ultrasound aided process, named as UASC and UPSC, had the yields of 81.53 and 94.88%, while ASC and PSC prepared with typical method showed the yields of 51.90 and 79.27%, respectively. All the collagens, with similar amino acid composition, contained alpha- and beta-chains and were type I. Based on circular dichroism (CD) and fourier transform infrared spectroscopy (FTIR), all the collagens had the triple helical structure. T-max of PSC and UPSC were higher than those of ASC and UASC. Overall, ultrasound treatment under appropriate condition effectively improved the extraction efficiency of ASC and PSC without affecting their physiochemical properties.