Direct methanol fuel cells have been attracting serious attention due to its high power density and low pollution, and huge effort has focused on its electrode catalysts. Here, a hydrothermal process was used to synthesize Pt/ three dimensional graphene (polyaniline) catalyst (Pt/3D-GNs (PANI)), in which PtCl42- and graphene oxide (GO) were reduced synchronously. Especially, aniline was polymerized to form polyaniline (PAM) in situ, catalyzed by pre-generated Pt. After cooling by liquid nitrogen, graphene sheets (GNs) were converted into three dimensional (3D) corrugated graphene. The Introduction of PANI promotes formation of 3D graphene structure and favors optimal distribution of Pt particles. Synergistic effect of 3D corrugated graphene structure, highly dispersive Pt nanopartilces and fast electron transfer of support makes Pt/3D-GNs (PANI) have superior electrocatalysis for methanol oxidation. Electrochemical tests show that Pt/3D-GNs (PANI) exhibits much higher electrocatalytic activity (1172 mA mg(-1)), better stability and improved CO tolerance in comparison with commercial Pt/C.