Intestinal permeability is the most important factor that determines the bioavailability. In-vitro techniques like Caco-2 model and Ussing chamber are used for evaluating the intestinal permeability of the bioactive compound. But the lack of mucosal layer in those models makes the intestinal absorption prediction less accurate. We developed an engineered small intestinal system to evaluate intestinal permeability of bioactive compound. Following this approach, we have evaluated the intestinal permeability of fat soluble and water soluble bioactive compound and compared with in situ single-pass intestinal perfusion technique. (C)2017 Elsevier Ltd. All rights reserved.