The encapsulation of fish oil in electrosprayed capsules using whey protein and carbohydrates (pullulan and dextran or glucose syrup) mixtures as glassy wall materials was studied. Capsules with fish oil emulsified by using only a rotor-stator emulsification exhibited higher oxidative stability than capsules where the oil was emulsified by high-pressure homogenization. Moreover, glucose syrup capsules (with a peroxide value, PV, of 19.7 +/- 4.4 meq/kg oil and a content of 1-penten-3-ol of 751.0 +/- 69.8 ng/g oil) were less oxidized than dextran capsules after 21 days of storage at 20 degrees C (PV of 24.9 +/- 0.4 meq/kg oil and 1-penten-3-ol of 1161.0 +/- 222.0 ng/g oil). This finding may be attributed to differences in oxygen permeability between both types of capsules. These results indicated the potential of both combinations of whey protein, pullulan, and dextran or glucose syrup as shell materials for the encapsulation of omega-3 PUFA in nano-microcapsules obtained by electrospraying. (C) 2018 Elsevier Ltd. All rights reserved.