Three-dimensional (3D) culture dish patterned with a microwell structure demonstrates a great application potential in biotechnology. This study reports on the easy fabrication of an ordered customizable honeycomb microwell array on the surface of polymer substrates including the commercial Petri dish to create a biological platform for cell culture. The fabrication method is based on a very simple solvent dip-coating technique and the methanol accumulation-induced phase separation in which a binary mixture of chloroform and methanol is used to induce a ternary solution and to guarantee the formation of the ordered pore array on the substrate. The surface topology of the honeycomb substrate is manipulated through varying the experimental conditions; notably, the obtained honeycomb structure is part of the substrate, which reveals an increase in the structure's stability for the practical applications. Honeycomb-structured Petri dish fabricated using this method is applied as a scaffold for cell growth to demonstrate its potential in biomedical applications. (C) 2017 Elsevier Inc. All rights reserved.