The synergistic wound healing and antibacterial activity of silver oxide nanoparticles embedded silk fibroin (Ag2O-SF) spuns is reported here. UV-Vis spectro photometric analysis of these spuns showed the surface plasmon resonance (SPR) confirming the formation of the silver oxide nanoparticles (Ag2O NPs) on the surface of the silk fibroin (SF). Scanning electron microscope (SEM) and Differential scanning calorimetry (DSC) also confirmed the presence of Ag2O NPs on surface of SF. X-ray diffraction (XRD) analysis revealed the crystalline nature of both SF and Ag2O-SF. Fourier transform infrared spectroscopy (FT-IR) results showed the different forms of silk (I and II) and their corresponding protein (amide I, II, Ill) confirmations. Biodegradation study revealed insignificant changes in the morphology of Ag2O-SF spuns even after 14 days of immersion in phosphate buffered saline (PBS). Ag2O-SF spuns showed excellent antibacterial activity against both pathogen (S. aureus and M. tuberculosis) and non-pathogen (E. coli) bacteria. More importantly, In vitro wound healing (scratch assay) assay revealed fast migration of the T3T fibroblast cells through the scratch area treated with extract of Ag2O-SF spuns and the area was completely covered within 24 h. Cytotoxicity assay confirmed the biocompatible nature of the Ag2O-SF spuns, thus suggesting an ideal material for wound healing and anti-bacterial applications. (C) 2017 Elsevier Inc. All rights reserved.