In this paper, porous microcapsules with tunable pore sizes were prepared using interfacial polymerization by employing a temperature-responsive cross-linking agent above its so-called cloud point temperature (T-scp). The influences of porosity on the surface morphology, release profile and biological activity of the microcapsules were investigated. The results showed that both pore size and pore density could be controlled by regulating either the amount of cross-linking agent or the ratio of core material to shell material. Furthermore, the porosity of the microcapsules determined their release properties and further regulated the bioactivity of the microcapsules. In addition, the mechanism of pore formation was confirmed by investigating the morphology of microcapsules below the T-scp The microencapsulation methodology described here is convenient and versatile, which can be easily extended to encapsulate a broad range of lipophilic core materials. (C) 2018 Elsevier Inc. All rights reserved.