Local single-phase flow heat transfer downstream a single pin fin in a microchannel was experimentally and numerically studied. Three distinct flow regimes, depending on the Reynolds number, were characterized, namely: laminar flow with steady wake, laminar flow with unsteady wake, and turbulent flow. Local temperature measurements with high spatial resolution were obtained by incorporating an array of micro resistance temperature detectors (RTDs) (similar to 55 mu m x 55 mu m) on the internal microchannel surface. Local surface temperatures were related to the flow structures under different flow regimes. An enhanced local heat transfer coefficient at the trailing edge of the wake region downstream the pillar was observed. It is believed to be a result of vortex shedding and large-scale flow mixing triggered by flow instability at high Reynolds number. The numerical model enabled a full conduction/convection conjugate analysis of the entire system including heat conduction within the solid substrates and heat losses to the surrounding environment. Local heat transfer coefficient downstream the pin fin at each Reynolds number was obtained. (C) 2018 Elsevier Ltd. All rights reserved.