In this work, a comprehensive experimental investigation on spray characteristics of four blended fuels, including diesel (D100), diesel-biodiesel (DB), diesel-biodiesel-ethanol (DBE), and diesel-biodiesel-diethyl ether (DBDE) has been conducted by using high pressure common rail injection system (up to 200 MPa). The transient spray behavior under various conditions was recorded by high speed photography with scattering light illumination. It is shown that higher injection pressure significantly accelerates the spray tip penetration (STP) evolution due to increased inertia of spray while increase in ambient pressure reduces the STP evolution due to higher gas resistance. With the addition of diethyl ether (DEE) into biodiesel, the STP of blended fuel tends to go down and corresponding projected area increases a lot when compared to DB. By means of particle droplet image analysis (PDIA) optical diagnostic method, spray microscopic parameters such as Sauter Mean Diameter (SMD), droplet diameter distribution probability curve, cumulative volume curve and characteristic diameter have been investigated. Results show that both the injection pressure and ambient pressure have significant influence on the spray microscopic characteristics. In addition, for fixed injection pressure and ambient pressure, when DEE is added into DB blends, the number fraction of smaller droplets increases, though the statistic diameter with peak probability is fixed at a certain value. Furthermore, SMD of the four tested fuels decreases sequentially in the order of DB, D100, DBE, and DBDE, indicating that DEE addition favors the atomization process.