Nano hydroxyapatite (Hap) was synthesized by green route using carrot peal extract and used as catalyst in thiophene oxidation reaction. The catalyst was characterized with TEM, XRD, XRF, SEM, BET and DLS size analysis. The particle size was found to be in the nano range. The highest conversion for thiophene oxidation was observed as 65%. Titanium and molybdenum were impregnated in nano Hap to investigate any further improvement of its efficiency towards the oxidation and a substantial increase in conversion of thiophene, 82%, was observed after impregnation. The influence of different process parameters on oxidation of thiophene using Ti-Mo impregnated nano Hap as catalyst was investigated. The metal-impregnated catalyst was characterized with FTIR, SEM, XRD, EDS analysis before and after the reaction. Langmuir-Hinshelwood (LH) model was proposed to explain the kinetics of the reaction.