Ir/Ce0.75Zr0.25O2 catalysts synthesized by the facile co-precipitation method were calcined at various temperatures. The influence of calcination temperature on their physicochemical features and catalytic behavior for carbon dioxide reforming of ethanol was investigated. Several key factors such as Ir dispersion, reducibility, and oxygen vacancies as well as Ir support interaction declined with increasing the calcination temperature, which result into the decrease of ethanol conversion and less intrinsic turnover frequency (TOF). Among the catalysts tested, the IrCeZr550 sample exhibited satisfactory activity and maintained stable performance as long as 90 h of time on-stream. In contrast, full ethanol conversion was only achieved at 750 degrees C for the more sintered IrCeZr850 catalyst, thereby higher than that of the others. Moreover, stability test of IrCeZr850 elucidated that ethanol conversion continuously decreased from 87% to 62%, and the molar ratio of H-2 to CO increased from 1.16 to 1.32 due to the inhibition of reverse water gas-shift reaction. For the deactivated IrCeZr850 catalyst, characterization results including HRTEM, XRD, Raman, and TPO revealed that the observed deactivation might be caused by the combination of the encapsulated carbon formation because of fewer oxygen defects and the remarkable sintering of active Ir species due to weaker Ir-support interaction. This established structure-activity relationship might provide insight on the development of suitable catalysts for syngas production from ethanol dry reforming.