The main objective of this work was the preparation, characterization and evaluation of membranes made from agave bagasse biomass through environmental friendly techniques. An Ethanosolv pretreatment (with four factors: temperature, ethanol, catalyst and residence time) was performed to recuperate cellulose using a central composite design 2 k + 2 k, k = 4 obtaining a response surface equation. The biomass obtained at maximal cellulose recovery was further acetylated by a Fisher process, in which the biomass reacted primarily with acetic acid. Afterwards, acetylated cellulignin membranes were produced by an evaporation-precipitation method. The resulting membranes (ABM) were characterized by SEM, AFM, FTIR, TGA and DMA, comparing with a CTA membrane obtained in the same conditions. Other membranes from cellulose obtained by acid-alkaline (AA), thermal (AT) and organosolv (AC) pretreatments were prepared. A comparison was made in terms of flux, fluoride rejection and acetylation yields. ABM achieved lower fluxes than AA, AT and AC. Only ABM rejected fluoride (98% removal for a 406 ppm solution). Afterwards, removal of the main cations and anions was assessed using ABM, from well water (Chihuahua, Mexico) which was as high as 99.99%. Biofouling was presented after 20 days of continuous operation. Agave bagasse, evaporation-precipitation method, acetylated cellulignin membranes, ion removal.