Cell surface-camouflaged graphene oxide immunosensor for identifying immune reactions

Yong Hwa Hwang; Eun Ae Jeon; Dong Yun Lee

Journal of Industrial and Engineering Chemistry, Vol.59, 28-34, March, 2018

DOI10.1016/j.jiec.2017.10.002 Export Citation

Cell surface-camouflaged graphene oxide immunosensor for identifying immune reactions

Yong Hwa Hwang^{1, 2}, Eun Ae Jeon^{1, 2}, and Dong Yun Lee^{1, 2, †}

¹Department of Bioengineering, College of Engineering, Hanyang University, Seoul 04763, Republic of Korea
²BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul 04763, Republic of Korea

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Monitoring the intensity of immune reactions is very useful to diagnose the fate of transplanted cells. For directly monitoring immune reactions after pancreatic islet cell transplantation, here graphene oxide (GO) quencher was chemically conjugated with fluorescence dye via Granzyme B (GrB)-specific peptide, so called to GO-based immunosensor. GO-based immunosensor was stably immobilized onto surface of living cells without damage of viability and functionality. The cell-surface camouflaged GO-based immunosensor could detect GrB (>2 unit/ml) secreted from immune cells, thereby dequenching fluorescence. Collectively, we anticipated that the GO-based immunosensor could be utilized to monitor cell graft rejection after cell therapy in vivo.

Keywords:Graphene oxide;Immunosensor;Cellular surface camouflage;Pancreatic islet;Granzyme B

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[2] Strober W, Watanabe T, Mucosal Immunol, 4, 484 (2011)

[3] Opelz G, Transplantation, 58, 443 (1994)

[4] Dreyer DR, Ruoff RS, Bielawski CW, Angew. Chem.-Int. Edit., 49, 9336 (2010)

[5] Loh KP, Bao Q, Eda G, Chhowalla M, Nat. Chem., 2, 1015 (2010)

[6] Huang PJJ, Liu J, Small, 8, 977 (2012)

[7] Sun X, Liu Z, Welsher K, Robinson J, Goodwin A, Zaric S, Dai H, Nano Res., 1, 203 (2008)

[8] Peng J, Gao W, Gupta BK, Liu Z, Romero-Aburto R, Ge L, Song L, Alemany LB, Zhan X, Gao G, Vithayathil SA, Kaipparettu BA, Marti AA, Hayashi T, Zhu JJ, Ajayan PM, Nano Lett., 12, 844 (2012)

[9] Peng C, Hu W, Zhou Y, Fan C, Huang Q, Small, 6, 1686 (2010)

[10] Zhang M, Yin BC, Wang XF, Ye BC, Chem. Commun., 47, 2399 (2011)

[11] Feng D, Zhang Y, Feng T, Shi W, Li X, Ma H, Chem. Commun., 47, 10680 (2011)

[12] Liao KH, Lin YS, Macosko CW, Haynes CL, ACS Appl. Mater. Interfaces, 3, 2607 (2011)

[13] Shapiro AMJ, Ricordi C, Hering BJ, Auchincloss H, Lindblad R, Robertson RP, Secchi A, Brendel MD, et al., N. Engl. J. Med., 355, 1318 (2006)

[14] Afonina IS, Cullen SP, Martin SJ, Immunol. Rev., 235, 105 (2010)

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[16] Hsieh MH, Nguyen HT, Int. Rev. Cytol., 245, 45 (2005)

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[18] Xi G, Wang X, Chen T, Int. J. Nanomed., 11, 1537 (2016)

[19] Piao Y, Liu F, Seo TS, Chem. Commun., 47, 14149 (2011)

[20] Kurschus FC, Fellows E, Stegmann E, Jenne DE, Proc. Natl. Acad. Sci. U. S. A., 105, 13799 (2008)

[21] Spaeny-Dekking EH, Hanna WL, Wolbink AM, Wever PC, Kummer JA, Swaak AJ, Middeldorp JM, Huisman HG, Froelich CJ, Hack CE, J. Immunol., 160, 3610 (1998)