In this paper amperometric glucose biosensors based on titanium (Ti) electrode coated with Prussian blue (PB) layer and immobilized glucose oxidase (GOx) is reported. Several techniques of PB layer deposition and immobilization of GOx were applied in order to find the most optimal design of biosensor. PB layer was formed by electrochemical PB deposition on titanium electrode in FeCl3 and K-3[Fe(CN)(6)] solution. GOx was immobilized on PB layer using two techniques: immobilization by electrochemical polymerization of pyrrole and fixation with vapor of glutaraldehyde. The designed biosensors displayed sensitivity toward glucose in the range from 0.19 to 3.87 mu A cm(-2) mM(-1). (c) 2017 The Electrochemical Society.