We report a regioregular bis-pyridal[2,1,3]thiadiazole (BPT) acceptor strategy to construct the first ambipolar pyridal[2,1,3]thiadiazole-based semiconducting polymer (PBPTV). The use of BPT unit enables PBPTV to achieve high electron affinity, low LUMO level, and extended pi-conjugation. All these factors provide PBPTV with encouraging hole and electron mobilities up to 6.87 and 8.49 cm(2) V-1 s(-1), respectively. Our work demonstrates that the BPT unit is a promising building block for designing high-performance electron-transporting semiconductors in organic electronics.