In this work, high purity amorphous silica derived from rice husk (RH) biomass was used to prepare green phosphor (Zn2SiO4:Mn2+). Based on the solid phase reaction under high temperature, the optimum doping concentration and reaction temperature were identified. The overall performance in terms of photoluminescence intensity and quantum yield of the RH-derived phosphor was superior to the one derived from commercial silica and close to the one made from silicic acid. The results showed that the phosphor derived from RH silica could serve as an alternative to commercial phosphor because of its decent properties and inexpensive green resource.