Pulsed laser welding of Ti alloy to stainless steel (SS) has been applied using pure V as an interlayer. Microstructures and mechanical properties of joints were improved by changing laser offset. Two-pass welding was applied, and two weld zones were produced in the joint. The unmelted V interlayer acted as a barrier to mixing of the two base materials during welding. The melting amount of base materials and V interlayer was controlled by changing laser offset to control the fusion ratio and thus to improve joint microstructure. sigma-Phase was present in V-SS weld zone of joint with laser offset of zero, which resulted in failure of the joint. Through changing laser offset, V concentration in V-SS weld zone of SS-0.2 mm offset joint was decreased and sigma-phase was eliminated in the joint. omega-Phase was discovered in Ti alloy-V weld zone of zero-offset joint, which had an adverse effect on mechanical property of the joint. Through changing laser offset, V concentration in Ti alloy-V weld zone of V-0.2 mm offset joint was increased and precipitation of omega-phase was prevented. Three joints (zero-offset joint, SS-0.2 mm offset joint and V-0.2 mm offset joint) exhibited the tensile strength of 504, 548 and 599 MPa, respectively.