A simple and inexpensive electrochemical methodology was used to develop a new method to inspect the biochemical action of Bleomycin (BLM). Furthermore, the effect of some metal ions such as Fe(II), Fe(IlI), Cu(I), Cu(II), Zn(II), Mn(II) and Co(Il) on the operation of BLM was investigated. To prove the role of ionic metals some chelators and reducers were used too. In this study, a pretreated pencil graphite electrode (PGE) modified with gold nanoparticles (AuNPs) and a synthesized zinc(II)-Schiff base complex (ZnSB) decorated with ds-DNA was used to identify DNA damage induced by BLM in the presence of metal ions. The change in the charge transfer resistance (R-ct) using EIS method as well as appearance of 8-Oxo guanine (8-OxoG) peak in DPV method after immersion of the DNA biosensor in BLM solution were used as indicators of DNA damage. Our results showed that the BLM alone cannot destroy DNA and in the other words the BLM has not any interaction with ds-DNA but BLM in the presence of some metal ions can destroy the ds-DNA. By comparing the performance of metal ions and investigation of their effect on action of BLM, the mechanism of DNA damage induced by BLM was presented.