Electrochemically reduced graphene oxide wrapped copper nanoparticles (ERGO/Cu NPs) composite were successfully synthesized through cyclic voltammetry. Highly stable, enhanced electroactive CuNPs growth was optimized by using different electrolyte solutions for deposition such as aqueous 0.1 M H3BO3 (I), 0.1 M H2SO4 (II) and 0.1 M Na2SO4 (III) containing 0.05 M CuSO4. Furtherly, electrocatalytic activity of the as prepared electrodes (I, II, III) were studied by non-enzymatic glucose oxidation. Obviously, lower over potential of glucose oxidation was noticed at ERGO/Cu NPs (I) modified electrode with amplified anodic current response compared than that of other electrodes (II, III). Then, ERGO/CuNPs (I) composite was characterized by SEM, FESEM, EDAX, XRD, Raman spectroscopy and cyclic voltammetry. In addition, ERGO/Cu NPs (I) modified electrode possess excellent electrocatalytic performance towards glucose in terms of wide linear range from 0.14 mu M to 5091 mM and lower limit of detection (LOD) 49 nM. Moreover, the fabricated sensor exhibits appreciable reproducibility, good stability and excellent selectivity. Furthermore, obtained real sample recovery results declare that the architecture sensor matrix could be a suitable platform for detection of glucose in human samples.