Biorefinery is the integration of various conversion and separation unit processes of biomass to energy, among other products. Downstream processes link these unit processes; however, these are often overlooked to affect energy yield. In this study, use of different alkaline agents and separation techniques, and order of operations, was assessed after conversion of processed sugar into hydrogen through dark fermentation. pH was adjusted to pH 6 using various basic agents; and vacuum filtration and centrifugation were performed to facilitate separation. Sugar loss of 7-40% due to the downstream processes was recorded; however, optimization of the processes ensured high volume and sugar recovery and low degradation byproduct production. Satisfactory volume recovery with high sugar and low byproduct concentrations were achieved after vacuum filtration and pH adjustment with aqueous base. H-2 yield and production rate significantly increased after performing the downstream processes. Peak H-2 production rate and yield were 1824 mL H-2 L-1 d(-1) and 1.27 mol H-2 mori sugar, respectively, for the optimum condition of vacuum filtration, followed by pH adjustment using 8 N Ca(OH)(2). (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.