The reaction rate of enolization of p-chloroacetophenone has been studied in the presence of amino acids, namely, beta-alanine, DL-alanine, L-alanine, and glycine. Maximum reaction rate was observed in the case of beta-alanine at 22M concentration. The effects of parameters such as effect of ketone concentration, effect of dielectric constant, effect of catalysts, etc., have been found to exert a significant effect on the reaction rate. The effect of temperature was studied in the range of 313-328 K, and several thermodynamic parameters such as entropy (Delta S-not equal), enthalpy (Delta S-not equal), energy of activation (Delta E-a), and Gibbs free energy (Delta F-not equal) were found to be -10.32 e.u., 17.87 cal mol(-1), 19.24 kcal mol(-1), and 21.20 cal mol(-1), respectively. The reaction rate increased from 1.9 to 5.8 min(-1) on increasing the percentage of dimethylformamide from 10 to 50% (v/v).