Psychological stress has recently been described as a risk factor in the development of pancreatic cancer. Here, we reported that increased neurotransmitter adrenaline was associated with the poor survival in pancreatic cancer patients. Moreover, in the cell model study, we found adrenaline promoted pancreatic cell PANG-1 migration in a dose dependent manner. Block of the (beta 2-adrenoreceptor with ICI118,551, significantly reduced cell migration. Further study found that adrenaline induced a cytoplasmic trans location of RNA binding protein HuR, which in turn activated TGF beta, as shown by the SBE luciferase assay and phosphorylation of Smad2/3. Either HuR knockdown or TGF beta inhibition reduced cell migration induced by adrenaline. Taken together, our study here revealed that adrenaline-HuR-TGF beta regulatory axis at least partially contributes to the psychological stress induced metastasis in PANC-1 cells, shedding light on therapeutic targeting psychological stress in improving the prognosis of pancreatic cancer. (C) 2017 Elsevier Inc. All rights reserved.