ELOVL3 is involved in elongating saturated and monounsaturated fatty acids, and is a critical enzyme for lipid accumulation in brown adipocytes during the early phase of tissue recruitment. In addition, ELOVL3 is related to increased fatty acid oxidation in brown adipocytes. However, the potential functions of ELOVL3 in bovine cells remain unclear. Herein, we aimed to elucidate the effect of the ELOVL3 on the differentiation of bovine skeletal muscle-derived satellite cells (MDSCs). Western blot and immunofluorescence analyses were used for elucidating ELOVL3 expression pattern in bovine MDSCs during differentiation in vitro. We activated or inhibited ELOVL3 to study the effect of alterations in its expression on in vitro differentiation of bovine MDSCs. ELOVL3 expression increased gradually during bovine MDSC differentiation, and its levels were higher in the more highly differentiated myotubes. Activation of ELOVL3 promoted MDSC differentiation, while inhibition of ELOVL3 hindered differentiation of these cells. Here, for the first time, we demonstrate the importance of ELOVL3 during bovine MDSC differentiation, which may assist in increasing beef cattle muscularity. (C) 2017 Published by Elsevier Inc.