Total internal reflection ellipsometry (TIRE) was utilized in its dynamic data acquisition mode to reveal the percentage of mercury present in an amalgam surface layer. In determining the optical constants of the amalgam film, the non-homogeneities of the formed surface layer were taken into account. The composition of the amalgam layer by percentage was determined using the EMA Bruggemann model for the analysis of the TIRE data. Regression results showed that amalgam layer consisted of mercury 16.00 +/- 0.43% and gold 84.00 +/- 0.43%. This real time TIRE analysis has shown that for these studies method can detect 0.6 +/- 0.4% of mercury on a gold surface, proving that this is a suitable optical technique for obtaining real time readouts. The structural analysis of SEM and AFM have shown that the amalgam layer had a dendritic structure, which formation was determined by the weak adhesion of the gold atoms onto its surface. (C) 2017 Elsevier B.V. All rights reserved.