Multifunctional biologically active materials have approached for antibiofilm, anticancer and osteoblast adhesion activities with significant biomedical applications, owing to this MWCNT modified with polypyrrole (PPy) matrix with the incorporation of palladium nanoparticles (NPs). The synthesized composite displays a tube-shaped morphology with highly dispersed crystalline Pd NPs, which are established through XRD, SEM, TEM and SAED studies. The pyridinic-N(similar to 402.7), pyrrolic N (similar to 400.8) peak in XPS spectra evidenced the interaction of PPy with Pd and MWCNT. Polymer stretching frequencies in FTIR and Raman spectroscopy proves successful formation of PPy and the Pd-N (1609 cm(-1)) interaction. In the stability aspect, it is up to 58.73% mass withstood at 800 degrees C in TGA analysis. The composite exhibits an efficient Anti-biofilm against a set of bacterial stain with planktonic cell growth. In vitro cytotoxicity of Vero and HeLa cell line assess the composites toxicity and anticancer activity up to 100 mu g. The outcome of cell adhesions showed that human osteosarcoma cells (HOS) can adhere and to develop on the MWCNT/PPy/Pd composites. Furthermore, the proliferation of cells on MWCNT/PPy/Pd composites was also proved the biocompatibility of the composites against HOS cells. These results suggest that Pd-doped MWCNT/PPy composites are promising materials for biomedical applications. (C) 2017 Elsevier B. V. All rights reserved.