Single walled carbon nanotube based biosensor for detection of peanut allergy-inducing protein ara h1

Abdus Sobhan; Jun-Hyun Oh; Mi-Kyung Park; Seung Wook Kim; Chulhwan Park; Jinyoung Lee

Korean Journal of Chemical Engineering, Vol.35, No.1, 172-178, January, 2018

DOI10.1007/s11814-017-0259-y Export Citation

Single walled carbon nanotube based biosensor for detection of peanut allergy-inducing protein ara h1

Abdus Sobhan, Jun-Hyun Oh, Mi-Kyung Park¹, Seung Wook Kim², Chulhwan Park^{3, †}, and Jinyoung Lee^†

Department of Plant and Food Sciences, Sangmyung University, 31 Sangmyungdae-gil, Dongnam-gu, Cheonan-si, Chungcheongnam-do 31066, Korea
¹School of Food Science and Biotechnology, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Korea
²Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea
³Department of Chemical Engineering, Kwangwoon University, 20 Gwangun-ro, Nowon-gu, Seoul 01897, Korea

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Food allergies are global trending issues in the food industry. Peanut allergy is an especially serious problem with emerging allergen symptoms because peanuts are utilized worldwide as a processing food source. Arachis hypogaea 1 (Ara h1) is a main seed storage protein from peanut materials that derives allergic medical symptoms. In this study, a single-walled carbon nanotube (SWCNT)-based biosensor was developed to detect Ara h1. The developed biosensor utilizes an Ara h1 antibody as a receptor for the target material, SWCNTs as a signal transfer, and 1- pyrenebutanoic acid succinimidyl ester (1-PBSE) as a linker between the SWCNTs and the receptors. SWCNTs dramatically transferred the biological reaction between the antibody and the antigen into measurable signals of electrical responses. The sensor capacity of the developed SWCNT-based biosensor, including the limit of detection (LOD, 1 ng/ mL), the detection range (1-1,000 ng/mL), and the washing amounts (three times), was confirmed.

Keywords:Single-walled Carbon Nanotube;Biosensor;Electrochemical Immunosensor;Food Allergy;Ara h1

[References]

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[Referenced By]

[1] Sicherer SH, Furlong AM, Godbold JH, Sampson HA, J. Allergy Clin. Immunol., 125, 1322 (2010)

[2] Grundy J, Matthews S, Bateman B, Dean T, Arshad SH, J. Allergy Clin. Immunol., 110, 784 (2002)

[3] Morisset M, Moneret-Vautrin DA, Kanny G, Eur. Ann. Allergy Clin. Immunol., 37, 54 (2005)

[4] Shek LP, Morales EAC, Soh SE, Gerez I, Ng PZ, Yi FC, Ma S, Lee BW, J. Allergy Clin. Immunol., 126, 324 (2010)

[5] Sun X, Guan L, Shan X, Zhang Y, Li Z, J. Agric. Food Chem., 60, 10979 (2012)

[6] Jong ECD, Zijverden MV, Spanhaak S, Koppelman SJ, Pellegrom H, Penninks AH, Clin. Exp. Allergy, 28, 743 (1998)

[7] Pomes A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD, J. Allergy Clin. Immunol., 111, 640 (2003)

[8] Besler M, Trac-Trend. Anal. Chem., 20, 662 (2001)

[9] Gaw A, Gourlay CW, Brown EA, Bell MA, Clin. Chim. Acta, 261, 175 (1997)

[10] Pollet J, Delport F, Janssen KPF, Tran DT, Wouters J, Verbiest T, Lammertyn J, Talanta., 83, 1436 (2011)

[11] Gupta V, Gulati P, Bhagat N, Dhar MS, Virdi JS, Eur. J. Clin. Microbiol., 34, 641 (2015)

[12] Thakur MS, Ragavan KV, J. Food Sci. Technol., 50, 625 (2013)

[13] Lazcka O, Campo FJD, Munoz FX, Biosens. Bioelectron., 22, 1205 (2007)

[14] Yamada K, Kim CT, Kim JH, Chung JH, Lee HG, Jun S, Plos One, 9, e10576 (2014)

[15] Allen BL, Kichambare PD, Star A, Adv. Mater., 19(11), 1439 (2007)

[16] Katz E, Willner I, Chem. Phys. Chem., 5, 1084 (2004)

[17] Besteman K, Lee JO, Wiertz FG, Heering HA, Dekker C, Nano Lett., 3, 727 (2003)

[18] Schasfoort RBM, Bergveld P, Kooyman RPH, Greve J, Anal. Chim. Acta, 238, 323 (1990)

[19] Jacobs CB, Peairs MJ, Venton BJ, Anal. Chim. Acta, 105, 662 (2010)

[20] Maehashi K, Katsura T, Kerman K, Takamura Y, Matsumoto K, Tamiya E, Anal. Chem., 79, 782 (2007)

[21] Aljaro CG, Cella LN, Shirale DJ, Park M, Munoz FJ, Yates MV, Mulchandani A, Biosens. Bioelectron., 26, 1437 (2010)

[22] Lee Y, Shin HY, Kang SW, Park C, Kim SW, Biosens. Bioelectron., 26, 2685 (2011)

[23] Zhang S, Huang TS, Bridgman R, Weese J, J. Food Sci., 71, M100 (2006)

[24] Zhou X, Park JY, Huang S, Liu J, McEuen PL, Phys. Rev. Lett., 95, 146805 (2005)

[25] Helbling T, Roman C, Hierold C, Nano Lett., 10, 3350 (2010)

[26] Wang J, Musameh M, Anal. Chem., 75, 2075 (2003)

[27] He P, Dai L, Mater. Res. Soc. Symp. P., 171, Springer US (2006).

[28] Alves RC, Pimentel FB, Nouws HP, Marques RC, Gonzalez-Garcia MB, Oliveira MBP, Delerue-Matos C, Biosens. Bioelectron., 64, 19 (2015)

[29] Villamizar RA, Maroto A, Rius FX, Inza I, Figueras MJ, Biosens. Bioelectron., 24, 279 (2008)

[30] Huang Y, Bell MC, Suni II, Anal. Chem., 80(23), 9157 (2008)