In the present work, we present the facial synthesis of reduced graphene oxide (Gr) intercalated-vanadium pentoxide (Gr-V2O5) nanosheets with varying wt % of graphene oxide (GO) (1 wt% - 5 wt%) into V2O5 layers. Physio-chemical properties of as prepared V2O5 and Gr-V2O5 nanosheets were characterized by XRD, XPS, Raman, FTIR, FESEM and HRTEM techniques. Results reveals that the Gr was effectively intercalated into V2O5 nanostructures and morphology of V2O5 was tuned from nanorods to nanosheets. Further, the Gr-V2O5 nanosheets on modified glassy carbon electrode (Gr-V2O5/GCE) was fabricated and utilized for electrochemical characterization. Under the optimized conditions, the modified electrodes is a simple and versatile tool for electrochemical detection of potentially hazardous 3,5- Dichlorophenol (DCp) with the wide concentration range between 1.9 x 10(-8) M to 2.2 x 10(-4) M. The modified electrode possesses highly selective and it can able to detect DCp at nanomolar concentration (5 nM) with a high sensitivity of 0.3577 mu A mu M-1. The modified electrode exhibits good reproducibility, high stability and also has promising electrochemical oxidation behavior of DCp in environmental samples even in presence of common interfering electroactive species. In view of analytical application, our proposed sensor could be directly employed for the electrochemical detection of DCp in industrial wastes, river water and sediment soil dispersion, without any pre- treatment. (C) 2017 Elsevier B.V. All rights reserved.