Herein, we report a novel positively charged photoluminescent Au-Ag bimetallic nanocluster synthesized using 11-mercaptoundecyl-N,N,N-trimethylammonium bromide as the capping ligand by means of green double-target sputtering method on a biocompatible polymer matrix. The photoluminescent Au-Ag bimetallic cluster showed emission tunability from blue to near infrared (NIR) regions with respect to change in the composition.