A re-generable spiral wound module of affinitive electrospun chitosan nanofiber membranes was fabricated, and the feasibility of the module for treating Cr (VI) contaminated water was studied. The effect of flow rate, initial Cr (VI) concentration, chitosan nanofiber deposition density, and other metal ions on Cr (VI) adsorption was investigated in detail. It was found that the loading capacity of the module was dependent on flow rate and nanofiber deposition density, but independent on initial Cr (VI) concentration. Lower flow rate led to higher adsorption capacity. The maximum adsorption capacity obtained with 2 g/m(2) nanofiber membranes in the module was 20.5 mg/g at 10% breakthrough. The module could also adsorb Cu (II), Cd (II) and Pb (II) ions separately but showed good selectivity to Cr (VI) when these metal ions were coexistent. The dynamic adsorption behavior of the module was better fitted by Dose-Response model. Furthermore, the separation of Cr (VI) by commercial nanofiltration (NF) membranes was examined and compared with the spiral wound nanofiber membranes constructed in this work. The result showed the latter had several advantages over the former in terms of rejection, flux and ease of operation.