A novel non-enzymatic glucose sensor based on SBA-15-supported copper ion (SBA-15-Cu (II)) nanomaterial was successfully prepared. The electrocatalytic activity of prepared modified electrode towards glucose oxidation was investigated by cyclic voltammetry and amperometric methods in alkaline solution. The amperometry results revealed that the prepared sensor exhibited a good response to glucose with a wide linear range from 0.5 mu M to 2 mM and with a low limit of detection of 0.075 mu M. The Nafion/SBA-15-Cu (II) modified glassy carbon electrode exhibited good reproducibility, long-term stability and negligible interference from uric acid, dopamine, and ascorbic acid. The Nafion/SBA-15-Cu (II) modified glassy carbon electrode showed good activity towards glucose oxidation in human serum samples with low overpotential, avoiding interference.