Hydrogen production from mesophilic anaerobic co-digestion of food waste (FW) and crude glycerol (GLC) was investigated in this study. Batch experiments were carried out at 35 degrees C for 36 h" to assess the effect of supplementation of different glycerol concentrations (1%, 3% and 5% (v/v)) on dark fermentation of FW. The maximum hydrogen yield (180 mLH(2)/gVS) was obtained at 5% GLC while the maximum specific production rate (around 13 mLH(2)/(gVS.h)) was similar for all experiments with glycerol addition. Besides contributing to increase H-2 productivity, the presence of glycerol reduced the microorganisms acclimation time (Lag phase) in comparison to the control tests conducted without this co-substrate. In addition, the increment of glycerol concentration also enhanced volatile fatty acids generation and favoured the production of 1,3-propanediol (1,3-PDO). In the experimental conditions studied (i.e., 1-5% (v/v) of GLC), the results revealed that co-digestion of FW and GLC is promising and can be potentially used to maximize energy production while contributing to the management and treatment of these wastes. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.